Review



caco 2 cells monolayer  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    ATCC caco 2 cells monolayer
    Effects of LGGp on gut barrier <t>integrity.</t> <t>Caco-2</t> cells were stimulated with 10 µg/ml LGGp for 48 (h) The TEER values were measured as follows: TEER = (measured resistance value−blank value) × single cell layer surface area (cm 2 ). The exposure to LGGp elicited a significant increase in TEER (A) . Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 h (B, C, D). FITC dextran permeability was assessed in transwell plate (B) and appeared reduced after 2 h in cells pre-treated with LGGp. Cells were processed for mRNA analysis by RT-PCR. Occludin (C) and ZO-1 (D) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucoronidase beta (GUS-B). Each point represents median and error with range (A, B) or median and interquartile range (C, D) of five independent experiments. Data were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; TEER, Trans-epithelial electrical resistance; ZO-1, zonula occludens 1. *p<0.05 vs NT.
    Caco 2 Cells Monolayer, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 16805 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/caco 2 cells monolayer/product/ATCC
    Average 99 stars, based on 16805 article reviews
    caco 2 cells monolayer - by Bioz Stars, 2026-02
    99/100 stars

    Images

    1) Product Images from "Postbiotic effects elicited by heat-inactivated Lacticaseibacillus rhamnosus GG against cow’s milk allergy in human cells"

    Article Title: Postbiotic effects elicited by heat-inactivated Lacticaseibacillus rhamnosus GG against cow’s milk allergy in human cells

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2025.1671729

    Effects of LGGp on gut barrier integrity. Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 (h) The TEER values were measured as follows: TEER = (measured resistance value−blank value) × single cell layer surface area (cm 2 ). The exposure to LGGp elicited a significant increase in TEER (A) . Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 h (B, C, D). FITC dextran permeability was assessed in transwell plate (B) and appeared reduced after 2 h in cells pre-treated with LGGp. Cells were processed for mRNA analysis by RT-PCR. Occludin (C) and ZO-1 (D) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucoronidase beta (GUS-B). Each point represents median and error with range (A, B) or median and interquartile range (C, D) of five independent experiments. Data were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; TEER, Trans-epithelial electrical resistance; ZO-1, zonula occludens 1. *p<0.05 vs NT.
    Figure Legend Snippet: Effects of LGGp on gut barrier integrity. Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 (h) The TEER values were measured as follows: TEER = (measured resistance value−blank value) × single cell layer surface area (cm 2 ). The exposure to LGGp elicited a significant increase in TEER (A) . Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 h (B, C, D). FITC dextran permeability was assessed in transwell plate (B) and appeared reduced after 2 h in cells pre-treated with LGGp. Cells were processed for mRNA analysis by RT-PCR. Occludin (C) and ZO-1 (D) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucoronidase beta (GUS-B). Each point represents median and error with range (A, B) or median and interquartile range (C, D) of five independent experiments. Data were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; TEER, Trans-epithelial electrical resistance; ZO-1, zonula occludens 1. *p<0.05 vs NT.

    Techniques Used: Permeability, Reverse Transcription Polymerase Chain Reaction, Expressing, Gene Expression, MANN-WHITNEY

    Effects of LGGp on human enterocytes differentiation. Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 (h) Cells were processed for mRNA analysis by RT-PCR. Muc2 (A) and Lactase (B) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucuronidase beta (GUS-B). Each point represents median and interquartile range of five independent experiments. Data were analyzed were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; Muc-2, mucin 2; *p<0.05 vs NT.
    Figure Legend Snippet: Effects of LGGp on human enterocytes differentiation. Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 (h) Cells were processed for mRNA analysis by RT-PCR. Muc2 (A) and Lactase (B) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucuronidase beta (GUS-B). Each point represents median and interquartile range of five independent experiments. Data were analyzed were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; Muc-2, mucin 2; *p<0.05 vs NT.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Expressing, Gene Expression, MANN-WHITNEY



    Similar Products

    caco 2 cells monolayer  (ATCC)
    99
    ATCC caco 2 cells monolayer
    Effects of LGGp on gut barrier <t>integrity.</t> <t>Caco-2</t> cells were stimulated with 10 µg/ml LGGp for 48 (h) The TEER values were measured as follows: TEER = (measured resistance value−blank value) × single cell layer surface area (cm 2 ). The exposure to LGGp elicited a significant increase in TEER (A) . Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 h (B, C, D). FITC dextran permeability was assessed in transwell plate (B) and appeared reduced after 2 h in cells pre-treated with LGGp. Cells were processed for mRNA analysis by RT-PCR. Occludin (C) and ZO-1 (D) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucoronidase beta (GUS-B). Each point represents median and error with range (A, B) or median and interquartile range (C, D) of five independent experiments. Data were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; TEER, Trans-epithelial electrical resistance; ZO-1, zonula occludens 1. *p<0.05 vs NT.
    Caco 2 Cells Monolayer, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/caco 2 cells monolayer/product/ATCC
    Average 99 stars, based on 1 article reviews
    caco 2 cells monolayer - by Bioz Stars, 2026-02
    99/100 stars
    		  Buy from Supplier
    human cell monolayers human colorectal adenocarcinoma derived caco 2 cells  (ATCC)
    99
    ATCC human cell monolayers human colorectal adenocarcinoma derived caco 2 cells
    Effects of LGGp on gut barrier <t>integrity.</t> <t>Caco-2</t> cells were stimulated with 10 µg/ml LGGp for 48 (h) The TEER values were measured as follows: TEER = (measured resistance value−blank value) × single cell layer surface area (cm 2 ). The exposure to LGGp elicited a significant increase in TEER (A) . Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 h (B, C, D). FITC dextran permeability was assessed in transwell plate (B) and appeared reduced after 2 h in cells pre-treated with LGGp. Cells were processed for mRNA analysis by RT-PCR. Occludin (C) and ZO-1 (D) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucoronidase beta (GUS-B). Each point represents median and error with range (A, B) or median and interquartile range (C, D) of five independent experiments. Data were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; TEER, Trans-epithelial electrical resistance; ZO-1, zonula occludens 1. *p<0.05 vs NT.
    Human Cell Monolayers Human Colorectal Adenocarcinoma Derived Caco 2 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human cell monolayers human colorectal adenocarcinoma derived caco 2 cells/product/ATCC
    Average 99 stars, based on 1 article reviews
    human cell monolayers human colorectal adenocarcinoma derived caco 2 cells - by Bioz Stars, 2026-02
    99/100 stars
    		  Buy from Supplier
    caco-2 cell monolayers  (Matos labs)
    90
    Matos labs caco-2 cell monolayers
    Effects of LGGp on gut barrier <t>integrity.</t> <t>Caco-2</t> cells were stimulated with 10 µg/ml LGGp for 48 (h) The TEER values were measured as follows: TEER = (measured resistance value−blank value) × single cell layer surface area (cm 2 ). The exposure to LGGp elicited a significant increase in TEER (A) . Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 h (B, C, D). FITC dextran permeability was assessed in transwell plate (B) and appeared reduced after 2 h in cells pre-treated with LGGp. Cells were processed for mRNA analysis by RT-PCR. Occludin (C) and ZO-1 (D) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucoronidase beta (GUS-B). Each point represents median and error with range (A, B) or median and interquartile range (C, D) of five independent experiments. Data were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; TEER, Trans-epithelial electrical resistance; ZO-1, zonula occludens 1. *p<0.05 vs NT.
    Caco 2 Cell Monolayers, supplied by Matos labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/caco-2 cell monolayers/product/Matos labs
    Average 90 stars, based on 1 article reviews
    caco-2 cell monolayers - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier
    caco-2 cell monolayer  (Matos labs)
    90
    Matos labs caco-2 cell monolayer
    Effects of LGGp on gut barrier <t>integrity.</t> <t>Caco-2</t> cells were stimulated with 10 µg/ml LGGp for 48 (h) The TEER values were measured as follows: TEER = (measured resistance value−blank value) × single cell layer surface area (cm 2 ). The exposure to LGGp elicited a significant increase in TEER (A) . Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 h (B, C, D). FITC dextran permeability was assessed in transwell plate (B) and appeared reduced after 2 h in cells pre-treated with LGGp. Cells were processed for mRNA analysis by RT-PCR. Occludin (C) and ZO-1 (D) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucoronidase beta (GUS-B). Each point represents median and error with range (A, B) or median and interquartile range (C, D) of five independent experiments. Data were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; TEER, Trans-epithelial electrical resistance; ZO-1, zonula occludens 1. *p<0.05 vs NT.
    Caco 2 Cell Monolayer, supplied by Matos labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/caco-2 cell monolayer/product/Matos labs
    Average 90 stars, based on 1 article reviews
    caco-2 cell monolayer - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier
    caco 2 cell monolayer  (DSMZ)
    96
    DSMZ caco 2 cell monolayer
    Effects of LGGp on gut barrier <t>integrity.</t> <t>Caco-2</t> cells were stimulated with 10 µg/ml LGGp for 48 (h) The TEER values were measured as follows: TEER = (measured resistance value−blank value) × single cell layer surface area (cm 2 ). The exposure to LGGp elicited a significant increase in TEER (A) . Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 h (B, C, D). FITC dextran permeability was assessed in transwell plate (B) and appeared reduced after 2 h in cells pre-treated with LGGp. Cells were processed for mRNA analysis by RT-PCR. Occludin (C) and ZO-1 (D) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucoronidase beta (GUS-B). Each point represents median and error with range (A, B) or median and interquartile range (C, D) of five independent experiments. Data were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; TEER, Trans-epithelial electrical resistance; ZO-1, zonula occludens 1. *p<0.05 vs NT.
    Caco 2 Cell Monolayer, supplied by DSMZ, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/caco 2 cell monolayer/product/DSMZ
    Average 96 stars, based on 1 article reviews
    caco 2 cell monolayer - by Bioz Stars, 2026-02
    96/100 stars
    		  Buy from Supplier
    caco 2 cell monolayers  (ATCC)
    99
    ATCC caco 2 cell monolayers
    Effects of LGGp on gut barrier <t>integrity.</t> <t>Caco-2</t> cells were stimulated with 10 µg/ml LGGp for 48 (h) The TEER values were measured as follows: TEER = (measured resistance value−blank value) × single cell layer surface area (cm 2 ). The exposure to LGGp elicited a significant increase in TEER (A) . Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 h (B, C, D). FITC dextran permeability was assessed in transwell plate (B) and appeared reduced after 2 h in cells pre-treated with LGGp. Cells were processed for mRNA analysis by RT-PCR. Occludin (C) and ZO-1 (D) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucoronidase beta (GUS-B). Each point represents median and error with range (A, B) or median and interquartile range (C, D) of five independent experiments. Data were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; TEER, Trans-epithelial electrical resistance; ZO-1, zonula occludens 1. *p<0.05 vs NT.
    Caco 2 Cell Monolayers, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/caco 2 cell monolayers/product/ATCC
    Average 99 stars, based on 1 article reviews
    caco 2 cell monolayers - by Bioz Stars, 2026-02
    99/100 stars
    		  Buy from Supplier
    the millicell–ers instrument used for measuring the transepithelial electrical resistance (teer) of the caco-2 cell monolayers  (Millipore)
    90
    Millipore the millicell–ers instrument used for measuring the transepithelial electrical resistance (teer) of the caco-2 cell monolayers
    Effects of LGGp on gut barrier <t>integrity.</t> <t>Caco-2</t> cells were stimulated with 10 µg/ml LGGp for 48 (h) The TEER values were measured as follows: TEER = (measured resistance value−blank value) × single cell layer surface area (cm 2 ). The exposure to LGGp elicited a significant increase in TEER (A) . Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 h (B, C, D). FITC dextran permeability was assessed in transwell plate (B) and appeared reduced after 2 h in cells pre-treated with LGGp. Cells were processed for mRNA analysis by RT-PCR. Occludin (C) and ZO-1 (D) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucoronidase beta (GUS-B). Each point represents median and error with range (A, B) or median and interquartile range (C, D) of five independent experiments. Data were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; TEER, Trans-epithelial electrical resistance; ZO-1, zonula occludens 1. *p<0.05 vs NT.
    The Millicell–Ers Instrument Used For Measuring The Transepithelial Electrical Resistance (Teer) Of The Caco 2 Cell Monolayers, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/the millicell–ers instrument used for measuring the transepithelial electrical resistance (teer) of the caco-2 cell monolayers/product/Millipore
    Average 90 stars, based on 1 article reviews
    the millicell–ers instrument used for measuring the transepithelial electrical resistance (teer) of the caco-2 cell monolayers - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier
    monolayer inflammatory barrier caco 2 cells  (ATCC)
    99
    ATCC monolayer inflammatory barrier caco 2 cells
    Effects of LGGp on gut barrier <t>integrity.</t> <t>Caco-2</t> cells were stimulated with 10 µg/ml LGGp for 48 (h) The TEER values were measured as follows: TEER = (measured resistance value−blank value) × single cell layer surface area (cm 2 ). The exposure to LGGp elicited a significant increase in TEER (A) . Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 h (B, C, D). FITC dextran permeability was assessed in transwell plate (B) and appeared reduced after 2 h in cells pre-treated with LGGp. Cells were processed for mRNA analysis by RT-PCR. Occludin (C) and ZO-1 (D) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucoronidase beta (GUS-B). Each point represents median and error with range (A, B) or median and interquartile range (C, D) of five independent experiments. Data were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; TEER, Trans-epithelial electrical resistance; ZO-1, zonula occludens 1. *p<0.05 vs NT.
    Monolayer Inflammatory Barrier Caco 2 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monolayer inflammatory barrier caco 2 cells/product/ATCC
    Average 99 stars, based on 1 article reviews
    monolayer inflammatory barrier caco 2 cells - by Bioz Stars, 2026-02
    99/100 stars
    		  Buy from Supplier
    caco-2 cell monolayers  (Pharmaron Inc)
    90
    Pharmaron Inc caco-2 cell monolayers
    Effects of LGGp on gut barrier <t>integrity.</t> <t>Caco-2</t> cells were stimulated with 10 µg/ml LGGp for 48 (h) The TEER values were measured as follows: TEER = (measured resistance value−blank value) × single cell layer surface area (cm 2 ). The exposure to LGGp elicited a significant increase in TEER (A) . Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 h (B, C, D). FITC dextran permeability was assessed in transwell plate (B) and appeared reduced after 2 h in cells pre-treated with LGGp. Cells were processed for mRNA analysis by RT-PCR. Occludin (C) and ZO-1 (D) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucoronidase beta (GUS-B). Each point represents median and error with range (A, B) or median and interquartile range (C, D) of five independent experiments. Data were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; TEER, Trans-epithelial electrical resistance; ZO-1, zonula occludens 1. *p<0.05 vs NT.
    Caco 2 Cell Monolayers, supplied by Pharmaron Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/caco-2 cell monolayers/product/Pharmaron Inc
    Average 90 stars, based on 1 article reviews
    caco-2 cell monolayers - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Effects of LGGp on gut barrier integrity. Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 (h) The TEER values were measured as follows: TEER = (measured resistance value−blank value) × single cell layer surface area (cm 2 ). The exposure to LGGp elicited a significant increase in TEER (A) . Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 h (B, C, D). FITC dextran permeability was assessed in transwell plate (B) and appeared reduced after 2 h in cells pre-treated with LGGp. Cells were processed for mRNA analysis by RT-PCR. Occludin (C) and ZO-1 (D) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucoronidase beta (GUS-B). Each point represents median and error with range (A, B) or median and interquartile range (C, D) of five independent experiments. Data were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; TEER, Trans-epithelial electrical resistance; ZO-1, zonula occludens 1. *p<0.05 vs NT.

    Journal: Frontiers in Immunology

    Article Title: Postbiotic effects elicited by heat-inactivated Lacticaseibacillus rhamnosus GG against cow’s milk allergy in human cells

    doi: 10.3389/fimmu.2025.1671729

    Figure Lengend Snippet: Effects of LGGp on gut barrier integrity. Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 (h) The TEER values were measured as follows: TEER = (measured resistance value−blank value) × single cell layer surface area (cm 2 ). The exposure to LGGp elicited a significant increase in TEER (A) . Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 h (B, C, D). FITC dextran permeability was assessed in transwell plate (B) and appeared reduced after 2 h in cells pre-treated with LGGp. Cells were processed for mRNA analysis by RT-PCR. Occludin (C) and ZO-1 (D) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucoronidase beta (GUS-B). Each point represents median and error with range (A, B) or median and interquartile range (C, D) of five independent experiments. Data were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; TEER, Trans-epithelial electrical resistance; ZO-1, zonula occludens 1. *p<0.05 vs NT.

    Article Snippet: For all experiments, we used a well validated model of gut barrier based on Caco-2 cells monolayer (American Type Culture Collection, Middlesex, UK; accession number: HTB-37) ( , ).

    Techniques: Permeability, Reverse Transcription Polymerase Chain Reaction, Expressing, Gene Expression, MANN-WHITNEY

    Effects of LGGp on human enterocytes differentiation. Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 (h) Cells were processed for mRNA analysis by RT-PCR. Muc2 (A) and Lactase (B) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucuronidase beta (GUS-B). Each point represents median and interquartile range of five independent experiments. Data were analyzed were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; Muc-2, mucin 2; *p<0.05 vs NT.

    Journal: Frontiers in Immunology

    Article Title: Postbiotic effects elicited by heat-inactivated Lacticaseibacillus rhamnosus GG against cow’s milk allergy in human cells

    doi: 10.3389/fimmu.2025.1671729

    Figure Lengend Snippet: Effects of LGGp on human enterocytes differentiation. Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 (h) Cells were processed for mRNA analysis by RT-PCR. Muc2 (A) and Lactase (B) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucuronidase beta (GUS-B). Each point represents median and interquartile range of five independent experiments. Data were analyzed were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; Muc-2, mucin 2; *p<0.05 vs NT.

    Article Snippet: For all experiments, we used a well validated model of gut barrier based on Caco-2 cells monolayer (American Type Culture Collection, Middlesex, UK; accession number: HTB-37) ( , ).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Gene Expression, MANN-WHITNEY